Journal: Nature Communications

Article Title: Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

doi: 10.1038/s41467-025-63993-z

Figure Lengend Snippet: a Sketch of an immunolabeled microtubule with primary and secondary antibodies. The secondary antibody is conjugated with a docking DNA strand complementary to the Cy3B-labeled imager strand. b Super-resolution and diffraction-limited images of the microtubule network of fixed U2OS cells obtained with the smartphone-based microscope. The super-resolved image represents the localization density, while the diffraction-limited image is the actual fluorescence intensity. Scale bar: 2 µm. c , d Comparison of super-resolved images of the microtubule network obtained with the smartphone-based setup ( c ) – a magnified image of the region marked in ( b ) - and with the high-end microscope ( d ). Scale bar: 2 µm. e Line profiles across super-resolved microtubules as indicated in ( c ) and ( d ). Black solid lines: smartphone-based microscope. Pink dashed lines: high-end microscope. In total, four different videos were obtained in which the immunolabeled microtubules could be resolved with the smartphone-based microscope. Source data are provided as a Source Data file.

Article Snippet: A human bone osteosarcoma epithelial (U2OS) cell culture with immunolabeled microtubules was purchased from Massive Photonics.

Techniques: Immunolabeling, Labeling, Microscopy, Fluorescence, Comparison